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Hoefer
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GenScript corporation
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WITec gmbh
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Medox Inc
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Avantor
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Fisher Bioreagents
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Fisher Scientific
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Eppendorf AG
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ICN Biomedicals
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Carl Roth GmbH
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Image Search Results
Journal: Frontiers in Physiology
Article Title: Myofibril and Mitochondrial Area Changes in Type I and II Fibers Following 10 Weeks of Resistance Training in Previously Untrained Men
doi: 10.3389/fphys.2021.728683
Figure Lengend Snippet: Electrophoresis, sarcoplasmic protein, and CS activity data. The figure in panel A is from SDS-PAGE; specifically, this figure demonstrates how the relative densities of myosin heavy chain and actin (lane 1 in this image) were quantified using band densitometry. Panel B shows how training affected relative myosin heavy chain (MyHC) and actin protein content per milligram wet muscle. Panel C shows how training affected sarcoplasmic protein concentrations per milligram wet muscle. Panel D shows how training affected CS activity levels, a surrogate of mitochondrial volume density. Bar graph data are presented as means ± standard deviation values, and individual participant data are overlaid. n =14 participants in the CS activity and sarcoplasmic protein panels, and n =15 participants in other panels.
Article Snippet: Briefly, SDS-PAGE preps (5μl) were loaded in duplicate on pre-casted gradient (4–15%) SDS-polyacrylamide gels (Bio-Rad Laboratories, Hercules, CA, United States) and subjected to electrophoresis at 200V for 40min using pre-made
Techniques: Electrophoresis, Activity Assay, SDS Page, Standard Deviation